Journal: bioRxiv

Article Title: Biophysical characterization reveals the similarities of liposomes produced using microfluidics and electroformation

doi: 10.1101/859124

Figure Lengend Snippet: (A) Design of the microfluidic chip used to produce the liposomes. The chip has three inlets for the inner (IA) and outer aqueous (OA) and the lipid-octanol (LO) phases, respectively. The vesicles are formed at a six-way junction (I) and flow along the channel to the outlet where they can be extracted or imaged directly. (B) Schematic of the OLA junction, where liposome formation occurs. The IA fluid stream is flanked by two channels with the lipid-carrying LO phase. The OA flows pinch off double emulsion droplets. The double emulsion self-assembles downstream into a vesicle with a 1-octanol pocket attached to it, which later buds off. We propose that the lipid ratio of the lipid-octanol mixture inserted into the microfluidic chip is maintained in the GUVs produced with OLA.

Article Snippet: The liquid flows were controlled with a pressure-driven microfluidic pump (MFCS-EZ, Fluigent, Le Kremlin-Bicêtre, France) equipped with a Fluiwell-4C reservoir kit.

Techniques: Liposomes, Double Emulsion, Produced

Journal: Biosensors

Article Title: Design, Simulation, and Evaluation of Polymer-Based Microfluidic Devices via Computational Fluid Dynamics and Cell Culture “On-Chip”

doi: 10.3390/bios13070754

Figure Lengend Snippet: Schematic representation of hepatocyte growth in microfluidic devices modeled and investigated using computational fluid dynamics tools. Image made in canva.com.

Article Snippet: The microfluidic experimental setup was composed of a chip and a microfluidic syringe pump (Aladin, World Precision Instruments, Tregoland OU, Tallinn, Estonia) for the accurate control and adjustment of fluid flow rates of media.

Techniques:

Journal: Biosensors

Article Title: Design, Simulation, and Evaluation of Polymer-Based Microfluidic Devices via Computational Fluid Dynamics and Cell Culture “On-Chip”

doi: 10.3390/bios13070754

Figure Lengend Snippet: 3D models of ( a ) circular and ( b ) elliptical designs and photographs of ( c ) circular and ( d ) elliptical microfluidic devices made of cyclic-olefin copolymer (COC) and polydimethylsiloxane (PDMS).

Article Snippet: The microfluidic experimental setup was composed of a chip and a microfluidic syringe pump (Aladin, World Precision Instruments, Tregoland OU, Tallinn, Estonia) for the accurate control and adjustment of fluid flow rates of media.

Techniques:

Journal: Biosensors

Article Title: Design, Simulation, and Evaluation of Polymer-Based Microfluidic Devices via Computational Fluid Dynamics and Cell Culture “On-Chip”

doi: 10.3390/bios13070754

Figure Lengend Snippet: 3D CAD models of the computational domain for ( a ) circular and ( b ) elliptical microfluidic devices.

Article Snippet: The microfluidic experimental setup was composed of a chip and a microfluidic syringe pump (Aladin, World Precision Instruments, Tregoland OU, Tallinn, Estonia) for the accurate control and adjustment of fluid flow rates of media.

Techniques:

Journal: Biosensors

Article Title: Design, Simulation, and Evaluation of Polymer-Based Microfluidic Devices via Computational Fluid Dynamics and Cell Culture “On-Chip”

doi: 10.3390/bios13070754

Figure Lengend Snippet: Live–dead cell imaging results of the Huh7 hepatoma cell line cultured in microfluidic devices in static and after 24 h of continuous flow: ( a ) circular and ( b ) elliptical microfluidic device in static mode ( left ) and after 24 h of continuous flow ( right ). Green (Calcein-AM)—live cells; red (Propidium Iodide)—dead cells.

Article Snippet: The microfluidic experimental setup was composed of a chip and a microfluidic syringe pump (Aladin, World Precision Instruments, Tregoland OU, Tallinn, Estonia) for the accurate control and adjustment of fluid flow rates of media.

Techniques: Imaging, Cell Culture

Journal: Biosensors

Article Title: Design, Simulation, and Evaluation of Polymer-Based Microfluidic Devices via Computational Fluid Dynamics and Cell Culture “On-Chip”

doi: 10.3390/bios13070754

Figure Lengend Snippet: ( a ) Albumin and ( b ) urea secretion levels produced by Huh7 cultured in the circular and elliptical microfluidic devices under continuous flow mode and in control samples cultured in static conditions.

Article Snippet: The microfluidic experimental setup was composed of a chip and a microfluidic syringe pump (Aladin, World Precision Instruments, Tregoland OU, Tallinn, Estonia) for the accurate control and adjustment of fluid flow rates of media.

Techniques: Produced, Cell Culture, Control